当前根据红细胞凝集（HA）滴度测量ABO抗体水平其可重复性较差，且不能良好的区别抗体浓度。因此我们发现了一种简单而快速的方法，采用流式细胞技术（FC）检测ABO抗体水平。为了证实该结果，我们共分析了79名血液供者的血清样本。IgM和IgG均被检测到，而IgG的检测仅限于O型血清的供者。1名O型患者在抗体清除后接受A2型供者移植后，我们收集了42例连续样本进行HA和FC分析。采用FC（相对中位荧光）检测所得的IgG的改变和HA的滴度明显相关，且重要的是相对中位荧光的升高先于排斥反应的发生。该方法能够检测ABO血型不容性移植相关的抗体水平的差异，且在目标物的检测和重复性来说相比于HA更有优势。

Current methods of measuring ABO antibody levels based on the hemagglutination (HA) titers have the disadvantages of relatively poor reproducibility and do not offer fine discrimination of antibody concentration. We therefore developed a simple and rapid method of measuring ABO antibody levels using flow cytometry (FC). For validation, we analyzed plasma samples from 79 blood donors. Both IgM and IgG were detected and measured with IgG essentially restricted blood group O donors. Forty-two successive samples were collected from a patient with blood group O undergoing antibody removal and subsequent transplantation from a group A2 donor and tested by both HA and FC. Changes in IgG measured by FC (relative median fluorescence) correlated well with HA titers and importantly rejection episodes were preempted by a rising relative median fluorescence. The method allowed quantitative discrimination in the range of antibody levels relevant to ABO incompatible transplantation and has the advantages over HA of objective measurement and reproducibility.