背景：如果没有怀孕、输血或者移植，健康人群一般不会出现HLA抗体。但是根据最新的研究我们发现无同种免疫的男性同样会出现HLA抗体。方法：424名健康男性供者采用Luminex但抗原磁珠进行检测。结果：以荧光值100作为标准，424名男性中有63%的患者检测到HLA抗体。42%的患者发现有I类抗体，12%的患者发现有II类抗体。抗体的特异性普通人群非常少见：18.9%的患者有A*3002抗体；超过10%的患者有A*3101, B76, B*8201, 和Cw*1701抗体。存在抗体的志愿者中大约有一半的志愿者有1种或2种特异性；另一半则存在有3种或者更多的特异性。在II类抗体中，20.5%存在DPA1*0201/DPB1*0101抗体, 10.8% 存在DQA1*0503/DQB1*0301抗体。由于上述数据424名志愿者均为墨西哥人，我们检测了洛杉矶的29名患者作为检验，结果发现抗体出现的频率相同。结果：健康男性存在特殊的HLA抗体类型。其原因可能是这些HLA抗体用于和微生物的抗原表位进行交叉反应，是摄入的蛋白和变应原使之成为自然抗体。

BACKGROUND: Human leukocyte antigen (HLA) antibodies were normally not found in subjects who have not been immunized by pregnancies, transfusions, or transplants. But with new methodology, we now see that HLA antibodies are often found in nonalloimmunized males. METHODS: The sera of 424 healthy male donors were tested with single antigen Luminex beads. RESULTS: Human leukocyte antigen antibodies were detected in 63% of 424 male blood donors when a fluorescent value of more than 1000 was used as the cutoff. Antibodies to class I was found in 42%, class II in 11% and both in 12%. Five males who were tested eight times over a 6-month period consistently had the same specificity at similar strength levels at each testing. The antibodies reacted with specificities that are rare in the general population: 18.9% had antibodies to A*3002; more than 10% had antibodies to A*3101, B76, B*8201, and Cw*1701. About half of the donors with antibodies had one or two specificities; the other half had three or more specificities. Among those with class II specificities, 20.5% had antibodies to DPA1*0201/DPB1*0101, and 10.8% to DQA1*0503/DQB1*0301. Because the above data were obtained by testing sera of 424 Mexican donors, as a check, 29 males in Los Angeles were tested and shown to have similar specificities at roughly similar frequencies. CONCLUSIONS: Normal males were found to have HLA antibodies to infrequent HLA specificities. It is likely that these HLA antibodies are produced to cross-reactive epitopes found in microorganisms, ingested proteins and allergens-making them natural antibodies.